Transgenesis & mouse genetics

Transgene expression in selected cell populations

 

Optogenetics, chemogenetics, & synaptic silencing

Interrogation and manipulation of cell/circuit activity

 

Viral-mediated genetic manipulations

Gene transfer and RNA interference

 

Anatomical circuit-mapping

Viral & conventional tracers mediated circuit architecture analysis

 

Ex vivo patch-clamp recording & fast-scan cyclic voltammetry

Examination of neurotransmission and cellular activity 

 

Single-cell RT-PCR & Patch-seq

Single-cell phenotypic and expression analyses

 

FACS-qPCR & TRAP-RNAseq

Transcriptomic analyses from anatomically/genetically-identified cell population

 

Immunohistochemistry & single molecule fluorescence in situ hybridizatio

Examination of transcript & protein expression in brain tissues

 

In vivo electrophysiology & fiber-photometry

Examination of cellular & circuit activity in anesthetized or behaving mice